Coding

Part:BBa_K2094002:Design

Designed by: Kyujin Jeong   Group: iGEM16_Korea_U_Seoul   (2016-10-07)


beta-agarase YM01-3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 794
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 810
    Illegal AgeI site found at 847
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was simply synthesized and cloned into pSB1C3


Source

This enzyme comes from Catenovulum agarivorans YM01. It was codon optimized for E.coli K-12 and was synthesized.

References